p p65 rabbit ab  (Bioss)

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-p65 Rabbit Ab , Bioss , bs-0982R , 1: 1500.

Techniques: Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

doi: 10.3389/fphar.2026.1655883

Figure Lengend Snippet: RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and p65 were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.

Article Snippet: After transfer, the membranes were blocked with sealing liquid and incubated for 2 h. Following blocking, membranes were incubated with primary antibodies against β-actin (dilution 1:1,000, ab8227, Abcam), ALIX (dilution 1:1,000, ab275377, Abcam), TSG101 (dilution 1:1,000, ab125011, Abcam), CD9 (dilution 1:1,000, ab236630, Abcam), Bax (dilution 1:1,000, ab32503, Abcam), Bcl-2 (dilution 1:1,000, ab32124, Abcam), Cleaved caspase 3 (dilution 1:500, ab32042, Abcam), TLR4 (dilution 1:1,000, TA382568, OriGene), TRIF (dilution 1:1,000, TA382523, OriGene), NF-κB p65 (dilution 1:1,000, TA385159, OriGene), Phospho-NF-κB p-p65 (dilution 1:1,000, TA380848, OriGene), STC1 (dilution 1:5000, ab229477, Abcam) at 4 °C overnight.

Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Western Blot

Journal: Poultry Science

Article Title: CSF3 enhances the innate immune responses to ALV-J infections via NF-κB and interferon pathways

doi: 10.1016/j.psj.2025.105648

Figure Lengend Snippet: CSF3 enhances the immune response to ALV-J via the NFκB signaling pathway. (A) Western blot analysis of p52, p100, phosphorylated p65 (p-p65), IκBα, and phosphorylated IκBα (p-IκBα) protein following CSF3 overexpression in DF-1 cells. (B) Quantification of protein levels in (A) based on relative grayscale values. (C) Western blot analysis of p52, p100, phosphorylated p65 (p-p65), IκBα, and phosphorylated IκBα (p-IκBα) protein following CSF3 knockdown in DF-1 cells. (D) Quantification of protein levels in (C) based on relative grayscale values. (D, E) RT-qPCR analysis of TNF-α, IL-1β , and IL-6 mRNA expression following CSF3 overexpression (D) or knockdown (E) in DF-1 cells. (F, G) ELISA measurement of TNF-α, IL-1β, and IL-6 protein levels following CSF3 overexpression (F) or knockdown (G) in DF-1 cells. (H, I) RT-qPCR analysis of TNF-α, IL-1β , and IL-6 mRNA expression following CSF3 overexpression (H) or knockdown (I) in CEF cells. (J, K) ELISA measurement of TNF-α, IL-1β, and IL-6 protein levels following CSF3 overexpression (J) or knockdown (K) in CEF cells. (L) Western blot analysis of STAT3, phosphorylated STAT3 (p-STAT3), env, β-actin, IκBα, p-IκBα, p-p65, p52, and p100 after STAT3 phosphorylation inhibition in CSF3-overexpressing DF-1 cells. (M) Quantification of protein levels in (L) based on relative grayscale values. Statistical significance was determined using a two-tailed unpaired Student’s t-test ( p < 0.05). * p < 0.05, **p < 0.01, *** p < 0.001.

Article Snippet: Mouse Anti-ALV-J envelope protein JE9 (kindly provided by Prof. Aijian Qin, Yangzhou University, Yangzhou, China), Rabbit Anti-STAT3 antibody (bs-1141R; Boss, China; 1:1000), Rabbit Anti-phospho-STAT3 (Ser727) antibody (bs-3429R; Boss, China; 1:1000), Rabbit Anti-NFκB2 antibody (10037P, Boss, China; 1:1000), Rabbit Anti-NFκB p-p65 (bs-0982R, Boss, China; 1:1000), Rabbit Anti-IKBα Rabbit (10268-1-AP, Proteintech, USA; 1:1000), Anti-p-IKBα (bs-2513R, Boss, China; 1:1000), and goat anti-rabbit IgG/HRP (bs13278), goat anti-mouse IgG/HRP (bs12478), goat Anti-Mouse IgG ( H + L ) FITC (bs10950) secondary antibody were purchased from Bioss (Beijing, China).

Techniques: Western Blot, Over Expression, Knockdown, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Inhibition, Two Tailed Test

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Rictor Ameliorates Acute Antibody-Mediated Rejection Following Kidney Transplantation by Suppressing Macrophage M1 Polarization Through p65-NLRP3 Axis.

doi: 10.1002/advs.202417119

Figure Lengend Snippet: Figure 7. Rictor inhibited NLRP3 transcription through increasing the K48-linked ubiquitination of p65 by E3 ubiquitin ligase SOCS1. a) Western blotting analysis of total and phosphorylated p65 in WT or Rictor-/-BMDMs treated with LPS (200 ng mL−1) for indicated time. b) Western blotting analysis of indicated proteins in WT or Rictor-/-BMDMs priming with LPS (200 ng mL−1) and JSH-23 (20 μm) for 4 h. c) qRT-PCR analysis showing the mRNA level of p65 in WT or Rictor-/- BMDMs treated with LPS (200 ng mL−1) for 4 h (n = 5). d) Luciferase activity in negative control (NC) or Rictor overexpression (OE) HEK293T cells transfected with plasmids encoding an NF-𝜅B luciferase reporter and TK-Renilla reporter, together with indicated plasmids, was measured

Article Snippet: The incubation was performed at 4 °C overnight with the primary antibodies against Rictor (1:1000, #2114, CST), Raptor (1:1000, #2280, CST), Akt (1:2000, #10176-2-AP, Proteintech), p-Akt(Ser473) (1:1000, #66444-1-Ig, Proteintech), pAkt(Thr308) (1:1000, #9275, CST), mTOR (1:1000, #2983, CST), p-mTOR (1:1000, #5536, CST), NLRP3 (1:1000, #15101, CST), IL-1β (1:1000, #31202, CST), Caspase-1 (1:1000, #24232, CST), cleaved-IL1β (1:1000, #63124, CST), cleaved-Caspase-1 (1:1000, #AG-20B-0042, AdigoGen), ASC (1:1000, #67824, CST), AIM2 (1:1000, #63660, CST), SOCS1 (1:1000, #55313, CST), p65 (1:1000, #8242, CST), p-p65 (1:1000, #TA325803, Origene), ubiquitin (#sc-8017, Santa Cruz), Flag (#14793, CST), HA (#51064-2-AP, Proteintech), Myc (#sc-40, Santa Cruz), GAPDH (1:10000, #60004-1-Ig, Proteintech) and β-actin (1:10000, #66009-1-Ig, Proteintech).

Techniques: Ubiquitin Proteomics, Western Blot, Quantitative RT-PCR, Luciferase, Activity Assay, Negative Control, Over Expression, Transfection